ANALYSIS OF MYELOPROTECTION EFFICIENCY OF ANTIOXIDANTS                                         IN EXPERIMENTAL CYTOSTATIC DAMAGE IN LEWIS LUNG CARCINOMA 

Minnigaleeva Sariet Dzhangaryevna, Assistant, sub-department of human physiology, Medical Institute, Penza State University (40 Krasnaya street, Penza, Russia), smin2005@yandex.ru
Mikulyak Nadezhda Ivanovna, Doctor of medical sciences, professor, head of sub-department of human physiology, Medical Institute, Penza State University (40 Krasnaya street, Penza, Russia), normphys@mail.ru
Magdeev Ruslan Ramisovich, Postgraduate student, Medical Institute, Penza State University (40 Krasnaya street, Penza, Russia), magdeev2008@rambler.ru
Mikulyak Artur Ivanovich, Cardiosurgeon, Federal Center of Cardio-Vascular Surgery (Penza) (6 Stasova street, Penza, Russia), mikulyak.artur@gmail.com
Ionicheva Lyubov' Vladimirovna, Candidate of medical sciences, associate professor, sub-department of human physiology, Medical Institute, Penza State University (40 Krasnaya street, Penza, Russia), normphys@mail.ru  

612.1.616-006-085 

Background. The purpose of the article is to study effectiveness of antioxidants’ influence such as mexidol, probucol and α-tocopherol on hematological parameters of the peripheral blood and bone marrow of mice with Lewis lung carcinoma in cytostatic therapy.
Materials and Methods. The syngeneic tumor system from the tumor strains bank of RCRC named after N.N. Blokhin of RAMS - Lewis lung carcinoma (LLC) served as a model of tumor growth. LLC tumor tissue was transplanted intramuscularly in the left paw thigh at amount of 1 × 106 cells in Hanks solution ("Biolot", Russia). Cyclophosphamide was introduced intraperitoneally to mice of line C57Bl / 6 in toxic dose of 100 mg / kg, starting from the 7th day after tumor inoculation, twice at an interval of 96 hours.
Results. Hematological analysis of peripheral blood showed that on the 14th day of the experiment in the process of tumor growth in mice with LLC there developed anemia and leukopenia with pronounced lymphopenia. Cyclophosphamide monotherapy aggravated the damage of myelocytic and erythrocytic germs of hematopoiesis with development of leukopenia and erythropenia. The number of leukocytes decreased mainly due to neutropenia, and on the 22nd day through the development of lymphopenia. Probucol and mexidol reduced hematotoxicity, reduced the damaging effect of cytostatics on granulocytopoiesis, hindered development of lymphopenia, protected the erythrocyte germ of hematopoiesis. This resulted in increasement of numbers of mature granulocytic cells, reduced the leukopenia deverity, and also increased the number of red blood cells and basophilic and polychromatic normocytes in bone marrow of animals.
Conclusion. The obtained results show effectiveness of the combined use of  mexidol, probucol, α-tocopherol with cyclophosphamide. Probucol and mexidol reduce hematotoxicity of the studied anticancer drugs. The α-tocopherol’s efficiency is lower than the efficiency of mexidol and probucol. 

myeloprotection efficiency, antioxidants, Lewis lung carcinoma. 

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